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Postnatal Development of the Craniofacial Skeleton in Male C57BL/6J Mice
For C57BL/6J male mice, postnatal day 60 is the earliest time point at which the skull achieves its adult shape and proportions. In addition, C57BL/6J male mice appear to have an inherent susceptibility to craniofacial malformation. Mice were housed in Optimice cages. Keywords: Anatomy, C57BL/6J, postnatal development, Optimice, mouse, mice Murat Maga, A. Postnatal Development…
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Efficient Chimeric Mouse Production Using a Novel Embryonic Stem Cell LIne
Researchers need an embryonic stem cell line that is able to participate in the formation of chimeric animals even after prolonged cultivation in vitro. A novel DGES1 embryonic stem cell line was demonstrated to efficiently produce transgenic mouse using the B6D2F1 blastocysts and CD-1 recipients. Mice were housed in Optimice cages. Keywords: Transgenic, Genetics, Gene…
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Combined Effects of Individual Culture and Atmospheric Oxygen on Preimplantation Mouse Embryos in Vitro
Atmospheric oxygen and individual culture are standard embryo culture conditions, the effect of combining them when investigated shows that cleavage timings, hatching rates and blastocyst cell numbers were reduced by either individual culture or atmospheric oxygen. Mice were housed in Optimice cages. Keywords: Transgenic, Genetics, Cell Culture, Culture media, Embryology, Preimplanation, mouse embryo group culture,…
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Addition of Interleukin-6 to Mouse Embryo Culture Increases Blastocyst Cell Number and Influences the Inner Cell Mass to Trophectoderm Ratio
These data show that IL-6 improved mouse embryo development when cultured individually in complex media; however, an excess of IL-6 may be detrimental. Mice were housed in Optimice cages. Keywords: Transgenic, Genetics, Cell culture, Culture media, Cytokines, Embryology, Fertilization in vitro, Preimplantation, Optimice, mice, mouse Rebecca L Kelley and David K Gardner. Addition of interleukin-6…
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Comparative Analysis of Pathologic Processes Developing in Mice Housed in SPF vs Non-SPF Conditions and Treated with Cyclophosphamide and dsDNA Preparation
CBA mice are treated with cyclophosphamide and fragmented exogenous dsDNA develop a very characteristic set of symptoms and 80–90% of such animals die within 6–25 days. Mice of the same strain housed under specific pathogen free (SPF) conditions do not develop the characteristic symptoms, nor do they become moribund. SPF mice were housed in Optimice…
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Homes for Animal Heroes: The truth has never felt so good! by Dr. Cindy Buckmaster
The Beagle Freedom Project (BFP) has been acquiring research dogs and using them as props to promote and fund a dangerous and misleading anti-research campaign, globally. Several research institutions in the U.S. have fallen prey to BFP’s dishonest tactics and have shut down their adoption programs to avoid further victimization by this animal rights group;…
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The Tree Shrew Life Picked Me! by Ms. Susan Freling
This seminar will take you on a journey through our world of working with the Northern Tree Shrew (Tupaia Belangeri). It’s not only interesting and informative, it’s exciting, fun and rarely a dull moment. Whether you’re a part of the husbandry or veterinary team, a researcher, or an IACUC member or administrator – you will…
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Bugs in the Mouse House-Expanding the Use of Arthropods in Biomedical Research by Dr. Bradley Ahrens
As the scientific community strives to reduce, refine, and replace animals used in research, there is an increasing demand to develop viable alternative models to meet the growing needs of modern science. To this end, invertebrate models are becoming increasingly prevalent, and present a useful alternative to mammals for studying human disease. To many, they…
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Challenges and Solutions for Laboratory Rodent Housing: presented by Dr. Joanna Makowska
What are rats and mice really like? What does it mean to provide laboratory rodents with a good life? The aim of this talk is to challenge existing preconceptions about what constitutes a “normal” laboratory rat or mouse. This lecture will showcase videos and data on laboratory rodents’ motivation to perform natural behaviors, focusing on…