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Morphophysiological Effects of Insertional Mutagenesis of the Contactin 5 (Cntn5) Gene in Transgenic Mice
The morphophysiological effects of integrating the transgenic construction providing the production of the human granulocyte–macrophage colony-stimulating factor in the milk of the mouse line (GM9) (the insertion of the construction in the intron of the contactin 5 (Cntn5) gene) isdescribed. Mice were housed in Optimice cages. Keywords: Metabolism, Transgenic, Gene Expression, insertional mutagenesis, fat metabolism,…
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Graft-Infiltrating PD-L1hi Cross-Dressed Dendritic Cells Regulate Antidonor T Cell Responses in Mouse Liver Transplant Tolerance “
The findings suggest that graft-infiltrating PD-L1hi CD-DCs may play a key role in the regulation of alloimmunity and in the induction of liver transplant tolerance.Mice were housed in Optimice cages. Keywords: Transgenic, Gene Expression, transplant rejection, liver transplant, organ transplant, C57BL/6, B6SJL-PtprcaPepcb/BoyJ, B6.Cg-Tg(Itgax-Venus)1Mnz/J, C3H/HeJ, Optimice, mouse, mice Ono, Y., Perez-Gutierrez, A., Nakao, T., Dai, H.,…
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Postnatal Development of the Craniofacial Skeleton in Male C57BL/6J Mice
For C57BL/6J male mice, postnatal day 60 is the earliest time point at which the skull achieves its adult shape and proportions. In addition, C57BL/6J male mice appear to have an inherent susceptibility to craniofacial malformation. Mice were housed in Optimice cages. Keywords: Anatomy, C57BL/6J, postnatal development, Optimice, mouse, mice Murat Maga, A. Postnatal Development…
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Food Deprivation Increases Hepatic Hepcidin Expression and Can Overcome the Effect of Hfe Deletion in Male Mice
These results indicate that Hamp1 induction by food deprivation is independent of HFE and suggest that targeting the pathway regulated by food deprivation could have clinical benefit in iron loading conditions. Mice were housed in Optimice cages. Keywords: Nutrition, Nutrional deficiencies, Transgenic, Gene Expression, food deprivation, hereditary hemochromatosis, Optimice, mouse, mice Cornel S. G. Mirciov,…
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Identification of a Glycogen Synthase Kinase-3β Inhibitor that Attenuates Hyperactivity in CLOCK Mutant Mice
Validating GSK-3β as a relevant therapeutic target in the identification of new therapies for bipolar patients. Mice were housed in Optimice cages. Keywords: Transgenic, Mental Health, Addiction, Gene Expression, CLOCK gene, Bipolar disorder, C57BL/6J, Optimice, mouse, mice Kozikowski, A. P., Gunosewoyo, H., Guo, S., Gaisina, I. N., Walter, R. L., Ketcherside, A., McClung, C. A.,…
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Effects of Female Sexual Chemosignals on Mucosal Immunity in BALB/c and C57BL/6 Male Mice
The sexual signals associated with infection risks provide genotype-dependent mobilization of the innate immunity without significant activation of physiological stress mechanisms. Mice were housed in Optimice cages. Keywords: Immune System, Immune response, female sexual chemosignals, lung mucosal immunity, Th1/Th2 immune balance, C57BL/6, BALB/c, Optimice, mouse, mice G. V. Kontsevaya, E. A. Litvinova, M. P. Moshkin.…
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Efficient Chimeric Mouse Production Using a Novel Embryonic Stem Cell LIne
Researchers need an embryonic stem cell line that is able to participate in the formation of chimeric animals even after prolonged cultivation in vitro. A novel DGES1 embryonic stem cell line was demonstrated to efficiently produce transgenic mouse using the B6D2F1 blastocysts and CD-1 recipients. Mice were housed in Optimice cages. Keywords: Transgenic, Genetics, Gene…
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Combined Effects of Individual Culture and Atmospheric Oxygen on Preimplantation Mouse Embryos in Vitro
Atmospheric oxygen and individual culture are standard embryo culture conditions, the effect of combining them when investigated shows that cleavage timings, hatching rates and blastocyst cell numbers were reduced by either individual culture or atmospheric oxygen. Mice were housed in Optimice cages. Keywords: Transgenic, Genetics, Cell Culture, Culture media, Embryology, Preimplanation, mouse embryo group culture,…
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Addition of Interleukin-6 to Mouse Embryo Culture Increases Blastocyst Cell Number and Influences the Inner Cell Mass to Trophectoderm Ratio
These data show that IL-6 improved mouse embryo development when cultured individually in complex media; however, an excess of IL-6 may be detrimental. Mice were housed in Optimice cages. Keywords: Transgenic, Genetics, Cell culture, Culture media, Cytokines, Embryology, Fertilization in vitro, Preimplantation, Optimice, mice, mouse Rebecca L Kelley and David K Gardner. Addition of interleukin-6…
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Behavioural Effects of Adult Vitamin D Deficiency in BALB/c Mice Are not Associated with Proliferation or Survival of Neurons in the Adult Hippocampus
This paper concludes that adult vitamin D deficiency was not associated with impaired proliferation or survival of adult born neurons in BALB/c mice and that the impact on rodent behaviour may not be due to altered neurogenesis per se, but to altered function of new hippocampal neurons or processes independent of adult neurogenesis. Mice were…